Structural interpretation of cryo-EM image reconstructions

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structural analysis of the spliceosomal complexes by cryo-EM

Keywords: membrane proteins; dynamic structure To understand the molecules we not only need to see how they look like, i.e. to determine their high-resolution static structures, we also need to know and understand how they move, we need to study their motions. In the course of our recent studies we have established an NMR spectroscopy-based approach to determine the dynamic structures of mammal...

متن کامل

Cross-validation in cryo-EM-based structural modeling.

Single-particle cryo-EM is a powerful approach to determine the structure of large macromolecules and assemblies thereof in many cases at subnanometer resolution. It has become popular to refine or flexibly fit atomic models into density maps derived from cryo-EM experiments. These density maps are typically significantly lower in resolution than electron density maps obtained from X-ray diffra...

متن کامل

Evolutionary tabu search strategies for the simultaneous registration of multiple atomic structures in cryo-EM reconstructions.

A structural characterization of multi-component cellular assemblies is essential to explain the mechanisms governing biological function. Macromolecular architectures may be revealed by integrating information collected from various biophysical sources - for instance, by interpreting low-resolution electron cryomicroscopy reconstructions in relation to the crystal structures of the constituent...

متن کامل

Component tree analysis of cystovirus φ6 nucleocapsid Cryo-EM single particle reconstructions

The 3-dimensional structure of the nucleocapsid (NC) of bacteriophage φ6 is described utilizing component tree analysis, a topological and geometric image descriptor. The component trees are derived from density maps of cryo-electron microscopy single particle reconstructions. Analysis determines position and occupancy of structure elements responsible for RNA packaging and transcription. Occup...

متن کامل

ResLog Plots: A New Metric for the Quality of Cryo-EM Reconstructions

1 Department of Chemistry and Biochemistry, 95 Chieftain Way, Florida State University, Tallahassee, FL 32306. 2 Institute of Molecular Biophysics, 91 Chieftain Way, Florida State University, Tallahassee, FL 32306. 3. Department of Physics, 77 Chieftan Way, Florida State University, Tallahassee, FL 32306-4350 4. Department of Biochemistry & Molecular Biology, School of Medicine, Oregon Health &...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Progress in Biophysics and Molecular Biology

سال: 2021

ISSN: 0079-6107

DOI: 10.1016/j.pbiomolbio.2020.07.004